Monitoring miRNA Expression and Activity in Mammalian Oocytes and Early Embryos

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F25%3A00645548" target="_blank" >RIV/68378050:_____/25:00645548 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/978-1-0716-4522-2_11" target="_blank" >https://doi.org/10.1007/978-1-0716-4522-2_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-4522-2_11" target="_blank" >10.1007/978-1-0716-4522-2_11</a>

Result language
angličtina
Original language name
Monitoring miRNA Expression and Activity in Mammalian Oocytes and Early Embryos
Original language description
MicroRNAs (miRNAs), genome-encoded small RNAs associated with Argonaute proteins, are important negative regulators of gene expression in mammalian cells. miRNAs usually partially base pair with mRNAs, suppress their translation, and destabilize them. Sufficient miRNA abundance is an important factor for efficient target repression. Experimental evidence suggests that oocyte growth causes a dilution effect, which reduces concentrations of maternal miRNAs and renders them functionally inefficient. Consequently, efficient target repression is retained only by those maternal miRNAs which achieve a favorable miRNA:mRNA stoichiometry. Here, we provide protocols for PCR-based quantification of miRNAs and luciferase reporter-based analysis of their activity in mammalian oocytes and early embryos.
Czech name
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Czech description
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Project
<a href="/en/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA for therapy</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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