Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00541492" target="_blank" >RIV/61388971:_____/21:00541492 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2076-2607/9/1/87" target="_blank" >https://www.mdpi.com/2076-2607/9/1/87</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9010087" target="_blank" >10.3390/microorganisms9010087</a>

Result language

angličtina

Original language name

Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis

Original language description

The expression of rRNA is one of the most energetically demanding cellular processes and, as such, it must be stringently controlled. Here, we report that DNA topology, i.e., the level of DNA supercoiling, plays a role in the regulation of Bacillus subtilis sigma(A)-dependent rRNA promoters in a growth phase-dependent manner. The more negative DNA supercoiling in exponential phase stimulates transcription from rRNA promoters, and DNA relaxation in stationary phase contributes to cessation of their activity. Novobiocin treatment of B. subtilis cells relaxes DNA and decreases rRNA promoter activity despite an increase in the GTP level, a known positive regulator of B. subtilis rRNA promoters. Comparative analyses of steps during transcription initiation then reveal differences between rRNA promoters and a control promoter, Pveg, whose activity is less affected by changes in supercoiling. Additional data then show that DNA relaxation decreases transcription also from promoters dependent on alternative sigma factors sigma(B), sigma(D), sigma(E), sigma(F), and sigma(H) with the exception of sigma(N) where the trend is the opposite. To summarize, this study identifies DNA topology as a factor important (i) for the expression of rRNA in B. subtilis in response to nutrient availability in the environment, and (ii) for transcription activities of B. subtilis RNAP holoenzymes containing alternative sigma factors.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA20-12109S" target="_blank" >GA20-12109S: The Unique Mycobacterial Transcription Machinery: Insights into its Composition, Structure & Functioning.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microorganisms

ISSN

2076-2607

e-ISSN

2076-2607

Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

CH - SWITZERLAND

Number of pages

17

Pages from-to

87

UT code for WoS article

000610593600001

EID of the result in the Scopus database

2-s2.0-85099025032