Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00550825" target="_blank" >RIV/61388971:_____/21:00550825 - isvavai.cz</a>

Result on the web

<a href="https://www.biomed.cas.cz/physiolres/pdf/2021/70_745.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2021/70_745.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934692" target="_blank" >10.33549/physiolres.934692</a>

Result language

angličtina

Original language name

Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro

Original language description

This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17 beta-estradiol and progesterone, and human transforming growth factor-beta 2 (TGF-beta 2) and TGF-beta 2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 mu g.ml(-1). It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17 beta-estradiol, progesterone, TGF-beta 2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 mu g.ml(-1), which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 mu g.ml(-1)) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

1802-9973

Volume of the periodical

70

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

745-754

UT code for WoS article

000729810300008

EID of the result in the Scopus database

2-s2.0-85122310132