Efficacy of Phytonutrients from Pomegranate Peel on Human Ovarian Cells in Vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918853" target="_blank" >RIV/62156489:43210/20:43918853 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.15414/jmbfs.2020.10.3.511-516" target="_blank" >https://doi.org/10.15414/jmbfs.2020.10.3.511-516</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2020.10.3.511-516" target="_blank" >10.15414/jmbfs.2020.10.3.511-516</a>

Result language

angličtina

Original language name

Efficacy of Phytonutrients from Pomegranate Peel on Human Ovarian Cells in Vitro

Original language description

Pomegranate fruit (Punica granatum L.) is rich in antioxidants with a content of bioactive substances with high medicinal value. Punicalagin, a polyphenol from pomegranate fruit, has been studied for its antioxidant, anti-proliferative and anti-cancer activities. Ovarian cancer is one of the most common cancers in the female reproductive organs and with high rate of lethality. While it is confirmed that pomegranate has significant beneficial effects on several types of cancer, there are few detailed reports on epithelial ovarian cancer. In accordance with the potential health-promoting effects of pomegranate, the aim of our study was to examine the in vitro effect of punicalagin and pomegranate peel extract at the different concentrations (12.5, 25, 50, 100, and 200 µg/mL) for 24 h on the human ovarian granulosa cell line HGL5 and human ovarian carcinoma cell line OVCAR-3. For this experiment, the ethanol extract from lyophilized pomegranate peel was prepared. The metabolic activity was determined by AlamarBlueTM cell viability assay, the secretion of steroid hormones was assayed by the ELISA method. The results showed a significant (P LESS-THAN OR EQUAL TO 0.001) decrease in the viability of HGL5 cells after the addition of the highest concentration of punicalagin (200 µg/mL). The number of viable OVCAR-3 cells was not significantly (P GREATER-THAN OR EQUAL TO 0.05) affected compared to the control. On the other hand, the concentrations 25, 50, 100, and 200 µg/mL of pomegranate peel extract led to a significant decrease in the viability of OVCAR-3 cells but did not cause any significant (P GREATER-THAN OR EQUAL TO 0.05) changes in the viability of HGL5. Although our studies revealed an increase in the release of 17β-estradiol levels by HGL5 cells after punicalagin treatment at the concentration 50 (P LESS-THAN OR EQUAL TO 0.01) and 100 (P LESS-THAN OR EQUAL TO 0.05) µg/mL, progesterone secretion was not significantly (P GREATER-THAN OR EQUAL TO 0.05) affected. Also, the release of 17ß-estradiol was significantly increased after the supplementation of pomegranate peel extract at the concentrations 50 (P LESS-THAN OR EQUAL TO 0.01), 100, and 200 (P LESS-THAN OR EQUAL TO 0.001) µg/mL. Furthermore, the levels of progesterone were significantly (P LESS-THAN OR EQUAL TO 0.05) decreased at concentrations 12.5, 25, 50, and 100 µg/mL. In conclusion, pomegranate phytonutrients might be a promising modulator of secretion of steroid hormones and it might serve to be a potential chemoprotective agent, reducing viability of ovarian cancer cells.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

30401 - Health-related biotechnology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

3

Country of publishing house

SK - SLOVAKIA

Number of pages

6

Pages from-to

511-516

UT code for WoS article

000600596600032

EID of the result in the Scopus database

2-s2.0-85098530533