Ovarian steroid hormone secretion by human granulosa cells after supplementation of sambucus nigra L. extract

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43920631" target="_blank" >RIV/62156489:43210/21:43920631 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.33549/physiolres.934680" target="_blank" >https://doi.org/10.33549/physiolres.934680</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934680" target="_blank" >10.33549/physiolres.934680</a>

Result language

angličtina

Original language name

Ovarian steroid hormone secretion by human granulosa cells after supplementation of sambucus nigra L. extract

Original language description

Beneficial effects of Sambucus nigra L. (black elder) as a traditional medicine have been associated with the phytoconstituents including polyphenols, terpenes and lectins. Various antioxidant rich natural products have also been implicated with improvement of reproductive health and fertility, however, the effect of Sambucus nigra on the ovarian cell functions has not been investigated yet. The objectives of the present study were to screen the polyphenols in the elderflower and elderberry extracts, and to examine the secretion activity of steroid hormones 17β-estradiol and progesterone by human ovarian granulosa cells HGL5 after supplementation of the extracts at a concentration range of 12.5 to 100 μg.ml-1. Qualitative as well as quantitative screening of polyphenols by high-performance liquid chromatography with diode-array detector (HPLC-DAD) analysis revealed rutin to be the most abundant polyphenol in both elderflower and elderberry extracts. In culture, neither elderflower nor elderberry extract caused any significant impact (p&gt;0.05) in cell viability as studied by AlamarBlue assay in comparison to control. However, a dosedependent stimulation of 17β-estradiol release was detected by ELISA after supplementation of elderflower (at 50 μg.ml-1; p&lt;0.01) and elderberry (at 100 μg.ml-1; p&lt;0.05) extracts at higher doses used in the study. On the other hand, both elderflower and elderberry extracts stimulated the secretion of progesterone by HGL5 cells at a lower dose (12.5 μg.ml-1; p&lt;0.05), as compared to control. Therefore, elderflower and elderberry extracts may have the potential to regulate steroidogenesis in ovarian cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30304 - Public and environmental health

Project

<a href="/en/project/8X20023" target="_blank" >8X20023: Impact of the digestion and absorption processes on the final biological activity of diet phytonutrients: real health added-value</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

—

Volume of the periodical

70

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

755-764

UT code for WoS article

000729810300009

EID of the result in the Scopus database

2-s2.0-85122334323