Changes in glycosyl inositol phosphoceramides during the cell cycle of the red alga Galdieria sulphuraria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00553480" target="_blank" >RIV/61388971:_____/22:00553480 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905645
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0031942221003745?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0031942221003745?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.phytochem.2021.113025" target="_blank" >10.1016/j.phytochem.2021.113025</a>
Alternative languages
Result language
angličtina
Original language name
Changes in glycosyl inositol phosphoceramides during the cell cycle of the red alga Galdieria sulphuraria
Original language description
Sphingolipids are significant component of plant-cell plasma membranes, as well as algal membranes, and mediate various biological processes. One of these processes is the change in lipid content during the cell cycle. This change is key to understanding cell viability and proliferation. There are relatively few papers describing highly glycosylated glycosyl inositol phosphorylceramide (GIPC) due to problems associated with the extractability of GIPCs and their analysis, especially in algae. After alkaline hydrolysis of total lipids from the red alga Galdieria sulphuraria, GIPCs were measured by highresolution tandem mass spectrometry and fragmentation of precursor ions in an Orbitrap mass spectrometer in order to elucidate the structures of molecular species. Fragmentation experiments such as tandem mass spectrometry in the negative ion mode were performed to determine both the ceramide group and polar head structures. Measurement of mass spectra in the negative regime was possible because the phosphate group stabilizes negative molecular ions [M-H]-. Analysis: of GIPCs at various stages of the cell cycle provided information on their abundance. It was found that, depending on the phases of the cell cycle, in particular during division, the uptake of all three components of GIPC, i.e., long-chain amino alcohols, fatty acids, and polar heads, changes. Structural modifications of the polar headgroup significantly increased the number of molecular species. Analysis demonstrated a convex characteristic for molecular species with only one saccharide (hexose or hexuronic acid) as the polar head. For two carbohydrates, the course of Hex-HexA was linear, while for HexA-HexA it was concave. The same was true for GIPC with three and four monosaccharides.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Phytochemistry
ISSN
0031-9422
e-ISSN
1873-3700
Volume of the periodical
194
Issue of the periodical within the volume
FEB 2022
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
113025
UT code for WoS article
000723417800001
EID of the result in the Scopus database
2-s2.0-85119909650