Tandem gene amplification restores photosystem II accumulation in cytochrome b(559) mutants of cyanobacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00553481" target="_blank" >RIV/61388971:_____/22:00553481 - isvavai.cz</a>
Result on the web
<a href="https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17785" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17785</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.17785" target="_blank" >10.1111/nph.17785</a>
Alternative languages
Result language
angličtina
Original language name
Tandem gene amplification restores photosystem II accumulation in cytochrome b(559) mutants of cyanobacteria
Original language description
Cytochrome (Cyt) b(559) is a key component of the photosystem II complex (PSII) that is essential for its proper functioning and assembly. Site-directed mutants of the model cyanobacterium Synechocystis sp. PCC6803 with mutated heme axial ligands of Cyt b(559) have little PSII and are therefore unable to grow photoautotrophically. Here we describe two types of Synechocystis autotrophic transformants that retained the same mutations in Cyt b(559) but are able to accumulate PSII and grow photoautotrophically. Whole-genome sequencing revealed that all of these autotrophic transformants carried a variable number of tandem repeats (from 5 to 15) of chromosomal segments containing the psbEFLJ operon. RNA-seq analysis showed greatly increased transcript levels of the psbEFLJ operon in these autotrophic transformants. Multiple copies of the psbEFLJ operon in these transformants were only maintained during autotrophic growth, while its copy numbers gradually decreased under photoheterotrophic conditions. Two-dimensional PAGE analysis of membrane proteins revealed a strong deficiency in PSII complexes in the Cyt b(559) mutants that was reversed in the autotrophic transformants. These results illustrate how tandem gene amplification restores PSII accumulation and photoautotrophic growth in Cyt b(559) mutants of cyanobacteria, and may serve as an important adaptive mechanism for cyanobacterial survival.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GX19-29225X" target="_blank" >GX19-29225X: Intertwined biogenesis of photosystems I and II: born together to work together</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
233
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
766-780
UT code for WoS article
000712906000001
EID of the result in the Scopus database
2-s2.0-85118240989