Flavonolignans from silymarin modulate antibiotic resistance and virulence in Staphylococcus aureus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00557462" target="_blank" >RIV/61388971:_____/22:00557462 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/22:43925352

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0753332222001949?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0753332222001949?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biopha.2022.112806" target="_blank" >10.1016/j.biopha.2022.112806</a>

Result language

angličtina

Original language name

Flavonolignans from silymarin modulate antibiotic resistance and virulence in Staphylococcus aureus

Original language description

Antibiotic resistance is currently a serious health problem. Since the discovery of new antibiotics no longer seems to be a sufficient tool in the fight against multidrug-resistant infections, adjuvant (combination) therapy is gaining in importance as well as reducing bacterial virulence. Silymarin is a complex of flavonoids and fla-vonolignans known for its broad spectrum of biological activities, including its ability to modulate drug resis-tance in cancer. This work aimed to test eleven, optically pure silymarin flavonolignans for their ability to reverse the multidrug resistance phenotype of Staphylococcus aureus and reduce its virulence. Silybin A, 2,3-dehydrosi-lybin B, and 2,3-dehydrosilybin AB completely reversed antibiotic resistance at concentrations of 20 mu M or less. Both 2,3-dehydrosilybin B and AB decreased the antibiotic-induced gene expression of representative efflux pumps belonging to the major facilitator (MFS), multidrug and toxic compound extrusion (MATE), and ATP-binding cassette (ABC) families. 2,3-Dehydrosilybin B also inhibited ethidium bromide accumulation and efflux in a clinical isolate whose NorA and MdeA overproduction was induced by antibiotics. Most of the tested flavonolignans reduced cell-to-cell communication on a tetrahydrofuran-borate (autoinducer-2) basis, with isosilychristin leading the way followed by 2,3-dehydrosilybin A and AB, which halved communication at 10 mu M. Anhydrosilychristin was the only compound that reduced communication based on acyl-homoserine lactone (autoinducer 1), with an IC50 of 4.8 mu M. Except for isosilychristin and anhydrosilychristin, all of the fla-vonolignans inhibited S. aureus surface colonization, with 2,3-dehydrosilybin A being the most active (IC50 10.6 mu M).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedicine & Pharmacotherapy

ISSN

0753-3322

e-ISSN

1950-6007

Volume of the periodical

149

Issue of the periodical within the volume

May 2022

Country of publishing house

FR - FRANCE

Number of pages

14

Pages from-to

112806

UT code for WoS article

000791274500002

EID of the result in the Scopus database

2-s2.0-85126617330