Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00580418" target="_blank" >RIV/61388971:_____/23:00580418 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10465518
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-3457-8_17" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-3457-8_17</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-3457-8_17" target="_blank" >10.1007/978-1-0716-3457-8_17</a>
Alternative languages
Result language
angličtina
Original language name
Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry
Original language description
Hydrogen/deuterium exchange (HDX) followed by mass spectrometry detection (MS) provides a fast, reliable, and detailed solution for the assessment of a protein structure. It has been widely recognized as an indispensable tool and already approved by several regulatory agencies as a structural technique for the validation of protein biopharmaceuticals, including antibody-based drugs. Antibodies are of a key importance in life and medical sciences but considered to be challenging analytical targets because of their compact structure stabilized by disulfide bonds and due to the presence of glycosylation. Despite these difficulties, there are already numerous excellent studies describing MS-based antibody structure characterization. In this chapter, we describe a universal HDX-MS workflow. Deeper attention is paid to sample handling, optimization procedures, and feasibility stages, as these elements of the HDX experiment are crucial for obtaining reliable detailed and spatially well-resolved information.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Mass Spectrometry-Based Proteomics
ISBN
978-1-0716-3456-1
Number of pages of the result
32
Pages from-to
303-334
Number of pages of the book
336
Publisher name
Humana
Place of publication
New York
UT code for WoS chapter
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