All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00583141" target="_blank" >RIV/61388971:_____/23:00583141 - isvavai.cz</a>

  • Result on the web

    <a href="https://link.springer.com/article/10.1007/s11626-023-00822-x" target="_blank" >https://link.springer.com/article/10.1007/s11626-023-00822-x</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11626-023-00822-x" target="_blank" >10.1007/s11626-023-00822-x</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study

  • Original language description

    In this work, we used splenocytes from healthy mice to study the effects of the two most commonly used cell culture media (A, B) with different compositions of redox reagents. The incubation of cells for 24 h resulted in a significant decrease in viability and metabolic activity of splenocytes, and the negative effects of incubation in medium B were more pronounced. In standard conditions, oxidative stress in cells was manifested by reduced mitochondrial potential, and this effect correlated with the transition of 58.3% of cells to the early stage of apoptosis under reducing conditions of medium A and up to 66.1% of cells under super-reducing conditions in medium B, suggesting altered cell physiology. High levels of ROS/RNS activated transcription factor Nrf2, superoxide dismutase 1, and catalase. The higher mRNA levels of these genes were under the conditions of medium B, whose super-reducing environment in combination with the environment of conventional incubators proved to be less suitable for the cells compared to medium A. Treatment of the cells with a lower concentration (10 mu g/ml) of oleoresin obtained from the microalga H. pluvialis partially eliminated the negative effects of cultivation. Higher concentration of oleoresin (40 mu g/ml) was slightly cytotoxic, due to the significant antioxidant effect of astaxanthin, the main bioactive component of the extract, which eliminated most of the ROS/RNS acting as signalling molecules. This study shows that the standard culture conditions do not reflect the physiological in vivo cell conditions, therefore, they are not generally suitable for incubation of all cell types.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    In Vitro - Cellular and Developmental Biology. Animal Section

  • ISSN

    1071-2690

  • e-ISSN

    1543-706X

  • Volume of the periodical

    59

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    764-777

  • UT code for WoS article

    001125836400002

  • EID of the result in the Scopus database

    2-s2.0-85178900103