Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00583141" target="_blank" >RIV/61388971:_____/23:00583141 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11626-023-00822-x" target="_blank" >https://link.springer.com/article/10.1007/s11626-023-00822-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11626-023-00822-x" target="_blank" >10.1007/s11626-023-00822-x</a>
Alternative languages
Result language
angličtina
Original language name
Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study
Original language description
In this work, we used splenocytes from healthy mice to study the effects of the two most commonly used cell culture media (A, B) with different compositions of redox reagents. The incubation of cells for 24 h resulted in a significant decrease in viability and metabolic activity of splenocytes, and the negative effects of incubation in medium B were more pronounced. In standard conditions, oxidative stress in cells was manifested by reduced mitochondrial potential, and this effect correlated with the transition of 58.3% of cells to the early stage of apoptosis under reducing conditions of medium A and up to 66.1% of cells under super-reducing conditions in medium B, suggesting altered cell physiology. High levels of ROS/RNS activated transcription factor Nrf2, superoxide dismutase 1, and catalase. The higher mRNA levels of these genes were under the conditions of medium B, whose super-reducing environment in combination with the environment of conventional incubators proved to be less suitable for the cells compared to medium A. Treatment of the cells with a lower concentration (10 mu g/ml) of oleoresin obtained from the microalga H. pluvialis partially eliminated the negative effects of cultivation. Higher concentration of oleoresin (40 mu g/ml) was slightly cytotoxic, due to the significant antioxidant effect of astaxanthin, the main bioactive component of the extract, which eliminated most of the ROS/RNS acting as signalling molecules. This study shows that the standard culture conditions do not reflect the physiological in vivo cell conditions, therefore, they are not generally suitable for incubation of all cell types.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
In Vitro - Cellular and Developmental Biology. Animal Section
ISSN
1071-2690
e-ISSN
1543-706X
Volume of the periodical
59
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
764-777
UT code for WoS article
001125836400002
EID of the result in the Scopus database
2-s2.0-85178900103