Comparison of holotomographic microscopy and coherence-controlled holographic microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00598790" target="_blank" >RIV/61388971:_____/24:00598790 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10487415

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/jmi.13260" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/jmi.13260</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jmi.13260" target="_blank" >10.1111/jmi.13260</a>

Result language

angličtina

Original language name

Comparison of holotomographic microscopy and coherence-controlled holographic microscopy

Original language description

Quantitative phase imaging (QPI) is a powerful tool for label-free visualisation of living cells. Here, we compare two QPI microscopes - the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope. Both systems provide unbiased information about cell morphology, such as individual cell dry mass, perimeter and area. The Q-Phase microscope uses artefact-free, coherence-controlled holographic imaging technology to visualise cells in real time with minimal phototoxicity. The 3D Cell Explorer-fluo employs laser-based holotomography to reconstruct 3D images of living cells, visualising their internal structures and dynamics. Here, we analysed the strengths and limitations of both microscopes when examining two morphologically distinct cell lines - the cuboidal epithelial MDCK cells which form multicellular clusters and solitary growing Rat2 fibroblasts. We focus mainly on the ability of the devices to generate images suitable for single-cell segmentation by the built-in software, and we discuss the segmentation results and quantitative data generated from the segmented images. We show that both microscopes offer slightly different advantages, and the choice between them depends on the specific requirements and goals of the user.nLabel-free imaging techniques have revolutionised how we study biological samples, allowing for in-depth analysis without staining or labelling. In this context, quantitative phase imaging (QPI) or Quantitative Phase Microscopy (QPM) has emerged as a powerful tool. Unlike traditional phase-contrast microscopy, which provides qualitative insights, QPI offers quantitative data with information about cell dry mass or cell volume. Here, we compare two advanced QPI microscopy systems: the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope for visualisation of two morphologically distinct cell lines. Each of these instruments comes with its strengths and limitations and different software solutions for detailed cell analysis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microscopy

ISSN

0022-2720

e-ISSN

1365-2818

Volume of the periodical

294

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

5-13

UT code for WoS article

001295203200001

EID of the result in the Scopus database

2-s2.0-85181914124