Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00599662" target="_blank" >RIV/61388971:_____/24:00599662 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/24:00599662 RIV/00216208:11110/24:10488389

Result on the web

<a href="https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.70004" target="_blank" >https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.70004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jev2.70004" target="_blank" >10.1002/jev2.70004</a>

Result language

angličtina

Original language name

Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells

Original language description

Escherichia coli A0 34/86 (EcO83) is a probiotic strain used in newborns to prevent nosocomial infections and diarrhoea. This bacterium stimulates both pro- and antiinflammatory cytokine production and its intranasal administration reduces allergicnairway inflammation inmice. Despite its benefits, there are concerns about the use of live probiotic bacteria due to potential systemic infections and gene transfer. Extracellular vesicles (EVs) derived from EcO83 (EcO83-EVs) might offer a safer alternative to live bacteria. This study characterizes EcO83-EVs and investigates their interaction with host cells, highlighting their potential as postbiotic therapeutics. EcO83-EVs were isolated, purified, and characterised following the Minimal Information of Studies of Extracellular Vesicles (MISEV) guidelines. Ex vivo studies conducted in human nasal epithelial cells showed that EcO83-EVs increased the expression of proteins linked to oxidative stress and inflammation, indicating an effective interaction between EVs and the host cells. Further in vivo studies in mice demonstrated that EcO83-EVs interact with nasal-associated lymphoid tissue, are internalised by airwaymacrophages, and stimulate neutrophil recruitment in the lung.Mechanistically, EcO83-EVs activate the NF-κΒ signalling pathway, resulting in the nitric oxide production. EcO83-EVs demonstrate significant potential as a postbiotic alternative to live bacteria, offering a safer option for therapeutic applications. Further research is required to explore their clinical use, particularly inmucosal vaccination and targeted immunotherapy strategies.n

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Extracellular Vesicles

ISSN

2001-3078

e-ISSN

2001-3078

Volume of the periodical

13

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

25

Pages from-to

e70004

UT code for WoS article

001336385000001

EID of the result in the Scopus database

2-s2.0-85206872579