Design and development of spectrophotometric enzymatic cyanide assays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00605222" target="_blank" >RIV/61388971:_____/25:00605222 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/25:00382235 RIV/00216208:11310/25:10495585
Result on the web
<a href="https://link.springer.com/article/10.1007/s00216-024-05703-0" target="_blank" >https://link.springer.com/article/10.1007/s00216-024-05703-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-024-05703-0" target="_blank" >10.1007/s00216-024-05703-0</a>
Alternative languages
Result language
angličtina
Original language name
Design and development of spectrophotometric enzymatic cyanide assays
Original language description
Determination of free cyanide (fCN) is required for various industrial, environmental, food, and clinical samples. Enzymatic methods are not widely used in this field despite their selectivity and mild conditions. Therefore, we present here a proof of concept for new spectrophotometric enzymatic assays of fCN. These are based on the hydrolysis of fCN affording the readily detectable NADH. fCN is hydrolyzed either in one step by cyanide dihydratase (CynD) or in two steps by cyanide hydratase (CynH) and formamidase (AmiF). An advantage of the latter route is the higher activity of CynH and AmiF compared to CynD. In both cases, the resulting formate is then transformed by an NAD-dependent formate dehydrogenase (FDH). The NADH thus formed is quantified colorimetrically using a known method based on a reduction of a tetrazolium salt (WST-8) with NADH. The developed assays of fCN are selective except for formic acid interference, proceed under mild conditions, and, moreover, fCN is detoxified during the reactions. The assays proceeded in a microtiter plate format. The limit of detection (LOD) and the limit of quantification (LOQ) were lower for the three-enzyme (CynH-AmiF-FDH) method (7.00 and 21.2 mu mol/L, respectively) than for the two-enzyme (CynD-FDH) method (10.7 and 32.4 mu mol/L, respectively). In conclusion, the new fCN assays presented in this work are selective, high-throughput, do not require harsh conditions, and use only small amounts of chemicals and enzymes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
1618-2650
Volume of the periodical
417
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
697-704
UT code for WoS article
001380457600001
EID of the result in the Scopus database
2-s2.0-85212503990