Enhancing the production of chlorophyll f in the cyanobacterium Synechocystis sp. PCC 6803
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00618539" target="_blank" >RIV/61388971:_____/25:00618539 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/ppl.70169" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ppl.70169</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.70169" target="_blank" >10.1111/ppl.70169</a>
Alternative languages
Result language
angličtina
Original language name
Enhancing the production of chlorophyll f in the cyanobacterium Synechocystis sp. PCC 6803
Original language description
One potential approach to improve the productivity of cyanobacteria and microalgae is to enhance photosynthetic efficiency by introducing far-red absorbing pigment molecules (such as chlorophylls f and d) into the photosynthetic apparatus to expand the range of photosynthetically active radiation. We have shown previously that expressing the ChlF subunit of Chroococcidiopsis thermalis PCC 7203 in the model cyanobacterium Synechocystis sp. PCC 6803 (Syn6803) is sufficient to drive the production of chlorophyll f (Chl f), but only to low levels (0.24% Chl f/Chl a). By using the strong Pcpc560 promoter and an N-terminal truncated derivative of ChlF, we have been able to increase the yield of Chl f in white light by over 30-fold to about 8.2% Chl f/Chl a, close to the level displayed by far-red photoacclimated C. thermalis 7203. Additionally, we demonstrate that ChlF from Fisherella thermalis PCC 7521, like ChlF from C. thermalis 7203, assembles into a variant of the monomeric photosystem II (PSII) core complex termed the super-rogue PSII complex when expressed in Syn6803. This contrasts with the originally reported formation of a ChlF homodimeric complex in Synechococcus sp. PCC 7002. Overall, our work is an important starting point for mechanistic and structural studies of super-rogue PSII and for incorporating Chl f into the photosynthetic apparatus of Syn6803.
Czech name
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Czech description
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Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004624" target="_blank" >EH22_008/0004624: PHOTOMACHINES - Photosynthetic cell redesign for high yields of therapeutic peptides</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiologia Plantarum
ISSN
0031-9317
e-ISSN
1399-3054
Volume of the periodical
177
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
Issue2
UT code for WoS article
001454111500001
EID of the result in the Scopus database
2-s2.0-105002037889