Utilization of high-resolution mass spectrometry and data-independent analysis to track the monoclonal antibody spatial stability

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00619327" target="_blank" >RIV/61388971:_____/25:00619327 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/25:10502969

Result on the web

<a href="https://www.tandfonline.com/doi/full/10.1080/14789450.2025.2492763" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/14789450.2025.2492763</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/14789450.2025.2492763" target="_blank" >10.1080/14789450.2025.2492763</a>

Result language

angličtina

Original language name

Utilization of high-resolution mass spectrometry and data-independent analysis to track the monoclonal antibody spatial stability

Original language description

Objectives: Chemical cross-linking coupled with mass spectrometry (CXMS) offers the distance constraints critical for building the structural model of protein and protein complexes and understanding dynamics of biological systems. Originally developed for protein structural models, CXMS has evolved into method for studying protein complex formation, ligand-induced conformational changes, and quantitative structural analysis using isotopically labeled cross-linkers. Methods: In this study we tested the potential of isotopically labeled MS-cleavable cross-linker to track the stability of the therapeutic monoclonal antibodies. Results: A novel isotopically labeled MS-cleavable cross-linker was synthesized, and its reactivity was successfully tested on peptide and protein standards. Further, the novel cross-linker was utilized to test the stability of selected therapeutical monoclonal antibodies, bevacizumab and trastuzumab, adopting the data-independent acquisition. Conclusion: This study reports the advantages of using combination of 13C isotopically labeled MS-cleavable cross-linkers and data-independent mass spectrometry analysis for the automated identification of cross-linked products and thus monitoring the structural rearrangement of protein structure.

Czech name

—

Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Expert Review of Proteomics

ISSN

1478-9450

e-ISSN

1744-8387

Volume of the periodical

22

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

199-207

UT code for WoS article

001471295500001

EID of the result in the Scopus database

2-s2.0-105003147151