Graphene oxide normal (GO + Mn2+) and ultrapure: Short‐term impact on selected antioxidant stress markers and cytokines in NHDF and A549 cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F21%3A00542566" target="_blank" >RIV/61388980:_____/21:00542566 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0319952" target="_blank" >http://hdl.handle.net/11104/0319952</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/antiox10050765" target="_blank" >10.3390/antiox10050765</a>
Alternative languages
Result language
angličtina
Original language name
Graphene oxide normal (GO + Mn2+) and ultrapure: Short‐term impact on selected antioxidant stress markers and cytokines in NHDF and A549 cell lines
Original language description
Since biological applications and toxicity of graphene‐based materials are structure dependent, studying their interactions with the biological systems is very timely and important. We studied short‐term (1, 24, and 48 h) effects of ultrapure (GO) and Mn2+ ‐contaminated (GOS) graphene oxide on normal human dermal fibroblasts (NHDF) and adenocarcinomic human alveolar basal epithelial cells (A549) using selected oxidative stress markers and cytokines: glutathione reductase (GR) and catalase (CAT) activity, total antioxidative capacity (TAC), and malondialdehyde (MDA) concentration, levels of vascular endothelial growing factor (VEGF), tumor necrosis factoralpha (TNF‐α), platelet‐derived growth factor‐BB (PDGF‐BB), and eotaxin. GOS induced higher levels of oxidative stress, measured with CAT activity, TAC, and MDA concentration than GO in both cell lines when compared to control cells. GR activity decreased in time in NHDF cells but increased in A549 cells. The levels of cytokines were related to the exposure time and graphene oxide type in both analyzed cell lines and their levels comparably increased over time. We observed higher TNF‐ α levels in NHDF and higher levels of VEGF and eotaxin in the A549 cell line. Both types of cells showed similar susceptibility to GO and GOS. We concluded that the short‐time exposure to GOS induced the stronger response of oxidative stress markers without collapsing the antioxidative systems of analysed cells. Increased levels of inflammatory cytokines after GO and GOS exposure were similar both in NHDF and A549 cells.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Antioxidants
ISSN
2076-3921
e-ISSN
2076-3921
Volume of the periodical
10
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
18
Pages from-to
765
UT code for WoS article
000653369000001
EID of the result in the Scopus database
2-s2.0-85105525541