Optogenetic confirmation of transverse-tubular membrane excitability in intact cardiac myocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388998%3A_____%2F24%3A00581733" target="_blank" >RIV/61388998:_____/24:00581733 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/24:00136200
Result on the web
<a href="https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP285202" target="_blank" >https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP285202</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1113/JP285202" target="_blank" >10.1113/JP285202</a>
Alternative languages
Result language
angličtina
Original language name
Optogenetic confirmation of transverse-tubular membrane excitability in intact cardiac myocytes
Original language description
T-tubules (TT) form a complex network of sarcolemmal membrane invaginations, essential for well-co-ordinated excitation-contraction coupling (ECC) and thus homogeneous mechanical activation of cardiomyocytes. ECC is initiated by rapid depolarization of the sarcolemmal membrane. Whether TT membrane depolarization is active (local generation of action potentials, AP) or passive (following depolarization of the outer cell surface sarcolemma, SS) has not been experimentally validated in cardiomyocytes. Based on the assessment of ion flux pathways needed for AP generation, we hypothesize that TT are excitable. We therefore explored TT excitability experimentally, using an all-optical approach to stimulate and record trans-membrane potential changes in TT that were structurally disconnected, and hence electrically insulated, from the SS membrane by transient osmotic shock. Our results establish that cardiomyocyte TT can generate AP. These AP show electrical features that differ substantially from those observed in SS, consistent with differences in the density of ion channels and transporters in the two different membrane domains. We propose that TT-generated AP represent a safety mechanism for TT AP propagation and ECC, which may be particularly relevant in pathophysiological settings where morpho-functional changes reduce the electrical connectivity between SS and TT membranes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physiology
ISSN
0022-3751
e-ISSN
1469-7793
Volume of the periodical
602
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
18
Pages from-to
791-808
UT code for WoS article
001160656600001
EID of the result in the Scopus database
2-s2.0-85185456991