Radioiodination and biodistribution of the monoclonal antibody TU-20 and its scFv fragment

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F10%3A00355466" target="_blank" >RIV/61389005:_____/10:00355466 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Radioiodination and biodistribution of the monoclonal antibody TU-20 and its scFv fragment
Original language description
The ability of the monoclonal antibody TU-20 and its scFv fragment to bind specifically to the C-end of the class III beta-tubulin makes these substances useful as potential diagnostics for neurodegenerative diseases-especially peripheral neuropathies. TU-20 and its scFv were labeled with I-125 and I-123 by chloramine-T (with radiochemical yield 75 and 50%, respectively). Radiochemical purity and stability was revealed by gel filtration (decrease to 80 and 50% in 2 months, respectively). Immunoreactivity of the labeled TU-20 was determined by ELISA-the range of the preserved immunoreactivity varies from 60 to 95% in accordance to the used radiolabeling process. RIA and affinity coupling analytic methods were specifically designed with focusing on specifics of the antibody and its fragment. The results of RIA differ in depandance on the type of the reaction vessel (glass or polystyrene) and the affinity coupling results depend on the experimental arrangement-in the batch or on the colum
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
<a href="/en/project/IBS1048301" target="_blank" >IBS1048301: Preparation of new radiopharmaceuticals based on monoclonal and recombinant antibodies and peptides labelled by alpha and beta radionuclides</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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