Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of Sc-44-DOTA-Puromycin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F13%3A00391532" target="_blank" >RIV/61389005:_____/13:00391532 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/content/pdf/10.1007%2Fs11307-012-0561-3" target="_blank" >http://link.springer.com/content/pdf/10.1007%2Fs11307-012-0561-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11307-012-0561-3" target="_blank" >10.1007/s11307-012-0561-3</a>
Alternative languages
Result language
angličtina
Original language name
Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of Sc-44-DOTA-Puromycin
Original language description
he purpose of this study was to investigate whether Sc-44-labeled puromycin can be utilized for imaging of protein synthesis in vivo. For micro-positron emission tomographic (mu PET) studies, 20-25 MBq of [Sc-44]-DOTA-puromycin was administered to tumor-bearing rats, and animals were scanned for 1 h dynamically. Results were further validated by dissecting organs and tissues of the animals after the measurement and in vitro blocking experiments using puromycin or cycloheximide to block protein synthesis. mu PET images of tumor-bearing rats showed significant tumor uptake of [Sc-44]-DOTA-puromycin and a clear-cut tumor visualization. In both blocking experiments, cellular uptake of [Sc-44]-DOTA-puromycin ([Sc-44]-DOTA-Pur) could be suppressed by blocking protein synthesis. We report for the first time successful mu PET imaging with Sc-44 obtained from a Ti-44/Sc-44 generator, as well as noninvasive mu PET imaging of ribosomal activity, respectively protein synthesis, with a puromycin-ba
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B06165" target="_blank" >2B06165: Production of positron emitting radionuclides Y-86 and I-124 for PET-imaging, study of their separation from the irradiated matrix and labelling of chosen prospective carriers</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Imaging and Biology
ISSN
1536-1632
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
79-86
UT code for WoS article
000314033000010
EID of the result in the Scopus database
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