Visualisation of in vivo protein synthesis during mycobacterial infection through [68Ga]Ga-DOTA-puromycin µPET/MRI
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F24%3A00598051" target="_blank" >RIV/61389005:_____/24:00598051 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1038/s41598-024-70200-4" target="_blank" >https://doi.org/10.1038/s41598-024-70200-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-024-70200-4" target="_blank" >10.1038/s41598-024-70200-4</a>
Alternative languages
Result language
angličtina
Original language name
Visualisation of in vivo protein synthesis during mycobacterial infection through [68Ga]Ga-DOTA-puromycin µPET/MRI
Original language description
Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct quantification of puromycin binding. This study assesses the value of [Ga-68]Ga-DOTA-puromycin in the visualisation of mycobacteria through positron emission tomography combined with magnetic resonance imaging (mu PET/MRI). The radiopharmaceutical was produced by previously published and validated methods. [Ga-68]Ga-DOTA-Puromycin imaging was performed on severe immunodeficient mice infected with acille Calmette-Guérin-derived M. Bovis (BCG). Acute and chronic infection stages were examined by mu PET/MRI. A follow-up group of animals acted as controls (animals bearing S. aureus-derived infection and sterile inflammation) to assess tracer selectivity. [Ga-68]Ga-DOTA-puromycin-mu PET/MRI images revealed the acute, widespread infection within the right upper shoulder and armpit. Also, [Ga-68]Ga-DOTA-puromycin signal sensitivity measured after a 12-week period was lower than that of [F-18]FDG-PET in the same animals. A suitable correlation between normalised uptake values (NUV) and gold standard histopathological analysis confirms accurate tracer accumulation in viable bacteria. The radiopharmaceutical showed infection selectivity over inflammation but accumulated in both M. Bovis and S. Aureus, lacking pathogen specificity. Overall, [Ga-68]Ga-DOTA-puromycin exhibits potential as a tool for non-invasive protein synthesis visualization, albeit without pathogen selectivity.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
19250
UT code for WoS article
001295308500120
EID of the result in the Scopus database
2-s2.0-85201534654