Coupled chemical reactions in dynamic nanometric confinement: VII. Biosensors based on swift heavy ion tracks with membranes
Result description
In previous papers it was shown that the coupling of the two chemical reactions: {NaOH etchant - PET polymer} and {NaOH etchant - AgNO3 solution} within the dynamic confinement of etched swift heavy ion tracks eventually leads to the formation of tiny Ag2O membranes within these nanopores, thus separating the latter ones into two adjacent segments. It is shown here that the deposition of enzymes in these two segments transforms these structures into biosensors. In our earlier developed sensors with transparent etched ion tracks, we frequently used glucose oxidase as enzyme and glucose as analyte. In these cases, the enzymatic reaction within the tracks leads to a change in the pH value of the confined solution and hence also in the track conductivity, so these structures can be used for biosensing. When applying, for easy comparison, the same enzyme/ analyte combination to the segmented sensor arrangement presented here, we find a striking improvement in detection sensitivity which points at a different biosensing mechanism due to intrinsic polarisation effects across the newly inserted membranes.
Keywords
The result's identifiers
Result code in IS VaVaI
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Coupled chemical reactions in dynamic nanometric confinement: VII. Biosensors based on swift heavy ion tracks with membranes
Original language description
In previous papers it was shown that the coupling of the two chemical reactions: {NaOH etchant - PET polymer} and {NaOH etchant - AgNO3 solution} within the dynamic confinement of etched swift heavy ion tracks eventually leads to the formation of tiny Ag2O membranes within these nanopores, thus separating the latter ones into two adjacent segments. It is shown here that the deposition of enzymes in these two segments transforms these structures into biosensors. In our earlier developed sensors with transparent etched ion tracks, we frequently used glucose oxidase as enzyme and glucose as analyte. In these cases, the enzymatic reaction within the tracks leads to a change in the pH value of the confined solution and hence also in the track conductivity, so these structures can be used for biosensing. When applying, for easy comparison, the same enzyme/ analyte combination to the segmented sensor arrangement presented here, we find a striking improvement in detection sensitivity which points at a different biosensing mechanism due to intrinsic polarisation effects across the newly inserted membranes.
Czech name
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Czech description
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Classification
Type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
GBP108/12/G108: Preparation, modification and characterization of materials by radiation
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Radiation Effects and Defects in Solids
ISSN
1042-0150
e-ISSN
—
Volume of the periodical
172
Issue of the periodical within the volume
1-2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
159-173
UT code for WoS article
000399481700018
EID of the result in the Scopus database
2-s2.0-85017588053
Basic information
Result type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
OECD FORD
Nano-materials (production and properties)
Year of implementation
2017