Length computation of irradiated plasmid DNA molecules

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F18%3A00499497" target="_blank" >RIV/61389005:_____/18:00499497 - isvavai.cz</a>

Alternative codes found

RIV/68378271:_____/18:00499497

Result on the web

<a href="http://dx.doi.org/10.1116/1.5050502" target="_blank" >http://dx.doi.org/10.1116/1.5050502</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1116/1.5050502" target="_blank" >10.1116/1.5050502</a>

Result language

angličtina

Original language name

Length computation of irradiated plasmid DNA molecules

Original language description

Compromised detection of short DNA fragments can result in underestimation of radiation-induced clustered DNA damage. The fragments can be detected with atomic force microscopy (AFM), followed by image analysis to compute the length of plasmid molecules. Plasmid molecules imaged with AFM are represented by open or closed curves, possibly with crossings. For the analysis of such objects, a dedicated algorithm was developed, and its usability was demonstrated on the AFM images of plasmid pBR322 irradiated with Co-60 gamma rays. The analysis of the set of the acquired AFM images revealed the presence of DNA fragments with lengths shorter than 300 base pairs that would have been neglected by a conventional detection method.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

<a href="/en/project/GJ17-03403Y" target="_blank" >GJ17-03403Y: Links between radiation track structure and clustered DNA damage</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biointerphases

ISSN

1934-8630

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

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UT code for WoS article

000454614500001

EID of the result in the Scopus database

2-s2.0-85059419374