How detection of plasmid DNA fragmentation affects radiation strand break yields

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00509919" target="_blank" >RIV/68378271:_____/19:00509919 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/19:00509919
Result on the web
<a href="https://doi.org/10.1093/rpd/ncy222" target="_blank" >https://doi.org/10.1093/rpd/ncy222</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/rpd/ncy222" target="_blank" >10.1093/rpd/ncy222</a>

Result language
angličtina
Original language name
How detection of plasmid DNA fragmentation affects radiation strand break yields
Original language description
A compromised detection of radiation-induced plasmid DNA fragments results in underestimation of calculated damage yields. Electrophoretic methods are easy and cheap, but they can only detect a part of the fragments, neglecting the shortest ones. These can be detected with atomic force microscopy, but at the expense of time and price. Both methods were used to investigate their capabilities to detect the DNA fragments induced by high-energetic heavy ions. The results were taken into account in calculations of radiation-induced yields of single and double strand breaks. It was estimated that the double strand break yield is twice as high when the fragments are at least partially detected with the agarose electrophoresis, compared to when they were completely omitted. Further increase by 13% was observed when the measured fragments were corrected for the fraction of the shortest fragments up to 300 base pairs, as detected with the atomic force microscopy. The effect of fragment detection on the single strand break yield was diminished.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)