Poly(HEMA) brushes emerging as a new platform for direct detection of food pathogen in milk samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F11%3A00362960" target="_blank" >RIV/61389013:_____/11:00362960 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/11:10304
Result on the web
<a href="http://dx.doi.org/10.1016/j.bios.2011.05.021" target="_blank" >http://dx.doi.org/10.1016/j.bios.2011.05.021</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2011.05.021" target="_blank" >10.1016/j.bios.2011.05.021</a>

Result language
angličtina
Original language name
Poly(HEMA) brushes emerging as a new platform for direct detection of food pathogen in milk samples
Original language description
The attachment of antibodies onto poly(HEMA) brushes prepared by surface initiated ATRP provided a successful way to fabricate SPR biosensors capable of in-real time detection Cronobacter at concentrations down to 106 cells mL1 in milk. Non-specific responses to deposition of non-targeted compounds ruled out other biosensors based on poly(MeOEGMA) and poly(HOEGMA) brushes or SAMs of EG2/COEG and EG6 from detection of Cronobacter at low concentrations even if the lowest detection limit of 104 cells mL1 in PBS was reached by biosensor based on SAM of EG6. Owing to well-controlled character of ATRP it was possible to minimize the thickness of the polymer brushes films so that the fouling could be still suppressed while the capture of antigens by antibodies immobilized on the brush layer could take place close to the gold SPR surface to provide a stronger optical response.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JA - Electronics and optoelectronics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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