Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00378735" target="_blank" >RIV/61389013:_____/12:00378735 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/jp303863q" target="_blank" >http://dx.doi.org/10.1021/jp303863q</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/jp303863q" target="_blank" >10.1021/jp303863q</a>
Alternative languages
Result language
angličtina
Original language name
Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study
Original language description
The entrapment of protein molecules in cubosomic nanocarriers that are sterically stabilized by an amphiphilic poly(ethylene glycol) (PEG) derivative are investigated. The mechanism of fragmentation of a self-assembled PEGylated cubic lipid phase into nanoparticles (NPs) is studied in excess aqueous medium. The molar ratio between the cubic-phase-forming lipid monoolein (MO) and its PEGylated derivative (MOPEG2000) is selected as to favor the formation of inverted-type liquid-crystalline (LC) structures. Freeze-fracture electron microscopy (FF-EM), quasi-elastic light scattering (QELS), confocal laser scanning fluorescence microscopy (CLSFM) and far-UV synchrotron radiation circular dichroism (SRCD) spectroscopy are applied for determination of the NPs' sizes, inner organization, stability, and interaction with the protein ?-chymotrypsinogen A. The PEGylated cubosomes offer new possibilities for investigation of protein loading in sterically stabilized (?Stealth) lipid carriers.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP208%2F10%2F1600" target="_blank" >GAP208/10/1600: Polymeric particles and nanostructured materials stabilized by surface active molecules</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physical Chemistry B
ISSN
1520-6106
e-ISSN
—
Volume of the periodical
116
Issue of the periodical within the volume
26
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
7676-7686
UT code for WoS article
000305933800009
EID of the result in the Scopus database
—