The evaluation of magnetic polymethacrylate-based microspheres used for solid phase DNA micro-extraction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00443087" target="_blank" >RIV/61389013:_____/15:00443087 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26310/15:PU113318 RIV/61989592:15110/15:33154935 RIV/00216305:26310/15:PU122750

Result on the web

<a href="http://dx.doi.org/10.3390/chromatography2020156" target="_blank" >http://dx.doi.org/10.3390/chromatography2020156</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/chromatography2020156" target="_blank" >10.3390/chromatography2020156</a>

Result language

angličtina

Original language name

The evaluation of magnetic polymethacrylate-based microspheres used for solid phase DNA micro-extraction

Original language description

Using magnetic particles as a solid-phase extraction system is the most frequently used micro-technique for DNA isolation. Particles with a complete covering of magnetic cores by a polymer are hence preferred. Quantitative polymerase chain reaction (qPCR) was used for the evaluation of the polymer coating efficiency of hydrophilic magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) and poly(glycidyl methacrylate) (PGMA) microspheres with/without carboxyl groups. The inhibition effect of magnetic microspheres was identified by the shift in Cq values (ΔCq) after the addition of different amounts of microspheres to PCR mixtures. With the increase of microsphere concentrations, the shift in Cq values to higher values was usually observed. P(HEMA-co-GMA) microspheres containing carboxyl groups extinguished the fluorescence at concentrations over 2 mg mL1 in a PCR mixture without any influence on the synthesis of PCR products. No PCR products (inhibition of DNA amplification) were detected in the presence of more than 0.8 mg mL1 in the PCR mixture of PGMA microspheres. Atomic force microscopy (AFM) was used for the determination of the surface morphology of the microspheres. The microspheres were spherical, and their surface was non-porous.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromatography

ISSN

2227-9075

e-ISSN

—

Volume of the periodical

2

Issue of the periodical within the volume

2

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

156-166

UT code for WoS article

—

EID of the result in the Scopus database

—