Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00463732" target="_blank" >RIV/61389013:_____/17:00463732 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.colsurfb.2016.10.007" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2016.10.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2016.10.007" target="_blank" >10.1016/j.colsurfb.2016.10.007</a>

Result language

angličtina

Original language name

Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

Original language description

Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals non-specifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-beta-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (gamma-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic gamma-Fe2O3 provides a relatively stable colloid product containing 48 mu mol of MCD g(-1). MCD-modified gamma-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of L-[C-14]glutamate and increase the extracellular L-[C-14]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Volume of the periodical

149

Issue of the periodical within the volume

1 January

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

64-71

UT code for WoS article

000390625200009

EID of the result in the Scopus database

2-s2.0-84994385928