Transient coating of .gamma.-Fe2O3 nanoparticles with glutamate for its delivery to and removal from brain nerve terminals
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00532093" target="_blank" >RIV/61389013:_____/20:00532093 - isvavai.cz</a>
Result on the web
<a href="https://www.beilstein-journals.org/bjnano/articles/11/122" target="_blank" >https://www.beilstein-journals.org/bjnano/articles/11/122</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3762/bjnano.11.122" target="_blank" >10.3762/bjnano.11.122</a>
Alternative languages
Result language
angličtina
Original language name
Transient coating of .gamma.-Fe2O3 nanoparticles with glutamate for its delivery to and removal from brain nerve terminals
Original language description
Glutamate is the main excitatory neurotransmitter in the central nervous system and excessive extracellular glutamate concentration is a characteristic feature of stroke, brain trauma, and epilepsy. Also, glutamate is a potential tumor growth factor. Using radiolabeled ʟ-[14C]glutamate and magnetic fields, we developed an approach for monitoring the biomolecular coating (biocoating) with glutamate of the surface of maghemite (γ-Fe2O3) nanoparticles. The nanoparticles decreased the initial rate of ʟ-[14C]glutamate uptake, and increased the ambient level of ʟ-[14C]glutamate in isolated cortex nerve terminals (synaptosomes). The nanoparticles exhibit a high capability to adsorb glutamate/ʟ-[14C]glutamate in water. Some components of the incubation medium of nerve terminals, that is, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and NaH2PO4, decreased the ability of γ-Fe2O3 nanoparticles to form a glutamate biocoating by about 50% and 90%, respectively. Only 15% of the amount of glutamate biocoating obtained in water was obtained in blood plasma. Albumin did not prevent the formation of a glutamate biocoating. It was shown that the glutamate biocoating is a temporal dynamic structure at the surface of γ-Fe2O3 nanoparticles. Also, components of the nerve terminal incubation medium and physiological fluids responsible for the desorption of glutamate were identified. Glutamate-coated γ-Fe2O3 nanoparticles can be used for glutamate delivery to the nervous system or for glutamate adsorption (but with lower effectiveness) in stroke, brain trauma, epilepsy, and cancer treatment following by its subsequent removal using a magnetic field. γ-Fe2O3 nanoparticles with transient glutamate biocoating can be useful for multifunctional theranostics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/GC20-02177J" target="_blank" >GC20-02177J: Antioxidant phenolic compound-modified magnetic nanoparticles for treatment of oxidative stress-related pathologies: Study of nano-biointerfaces</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Beilstein Journal of Nanotechnology
ISSN
2190-4286
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
10 Sep
Country of publishing house
DE - GERMANY
Number of pages
13
Pages from-to
1381-1393
UT code for WoS article
000577419700001
EID of the result in the Scopus database
2-s2.0-85091828008