Scavenging of reactive oxygen species by phenolic compound-modified maghemite nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00504705" target="_blank" >RIV/61389013:_____/19:00504705 - isvavai.cz</a>
Result on the web
<a href="https://www.beilstein-journals.org/bjnano/content/pdf/2190-4286-10-108.pdf" target="_blank" >https://www.beilstein-journals.org/bjnano/content/pdf/2190-4286-10-108.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3762/bjnano.10.108" target="_blank" >10.3762/bjnano.10.108</a>
Alternative languages
Result language
angličtina
Original language name
Scavenging of reactive oxygen species by phenolic compound-modified maghemite nanoparticles
Original language description
Maghemite (γ-Fe2O3) nanoparticles obtained through co-precipitation and oxidation were coated with heparin (Hep) to yield γ-Fe2O3@Hep, and subsequently with chitosan that was modified with different phenolic compounds, including gallic acid (CS-G), hydroquinone (CS-H), and phloroglucinol (CS-P), to yield γ-Fe2O3@Hep-CS-G, γ-Fe2O3@Hep-CS-H, and γ-Fe2O3@Hep-CS-P particles, respectively. Surface modification of the particles was analyzed by transmission electron microscopy, dynamic light scattering, attenuated total reflection Fourier transform infrared spectroscopy, and thermogravimetric analysis. Magnetic measurements indicated that the polymer coating does not affect the superparamagnetic character of the iron oxide core. However, magnetic saturation decreased with increasing thickness of the polymer coating. The antioxidant properties of the nanoparticles were analyzed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Cellular uptake and intracellular antioxidant activity of the particles were evaluated by an iron assay and flow cytometry, respectively, using L-929 and LN-229 cells. Compared to the control, the phenolic modification significantly reduced intracellular reactive oxygen species (ROS) levels to 35–56%, which was associated with a 6–8-times higher cellular uptake in L-929 cells and a 21–31-times higher cellular uptake in LN-229 cells. In contrast, γ-Fe2O3@Hep particles induced a 3.8-times and 14.9-times higher cellular uptake without inducing antioxidant activity. In conclusion, the high cellular uptake and the antioxidant properties associated with the phenolic moieties in the modified particles allow for a potential application in biomedical areas.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/GC16-01128J" target="_blank" >GC16-01128J: Antioxidative magnetic nanoparticles based on natural antioxidants: Nanoparticle-cell interactions</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Beilstein Journal of Nanotechnology
ISSN
2190-4286
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
20 May
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
1073-1088
UT code for WoS article
000468725700002
EID of the result in the Scopus database
2-s2.0-85073539823