Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00506160" target="_blank" >RIV/61389013:_____/19:00506160 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/19:PU136161
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/17435390.2018.1555624" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/17435390.2018.1555624</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/17435390.2018.1555624" target="_blank" >10.1080/17435390.2018.1555624</a>
Alternative languages
Result language
angličtina
Original language name
Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine
Original language description
Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12 nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles. Conventional gama-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25 pg Fe/cell for HA-PEG@Fe3O4,gama-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12–75 μg/cm2) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gama-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 μg/cm2, 72 h) and also decreased the phagocytic activity of monocytes (15 μg/cm2 24 h, 3–75 μg/cm2 72 h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nanotoxicology
ISSN
1743-5390
e-ISSN
—
Volume of the periodical
13
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
510-526
UT code for WoS article
000477976100006
EID of the result in the Scopus database
2-s2.0-85060941650