Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00458566" target="_blank" >RIV/61389013:_____/16:00458566 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity

Original language description

To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CD - Macromolecular chemistry

OECD FORD branch

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Project

<a href="/en/project/GC16-01128J" target="_blank" >GC16-01128J: Antioxidative magnetic nanoparticles based on natural antioxidants: Nanoparticle-cell interactions</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Croatian Medical Journal

ISSN

0353-9504

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

2

Country of publishing house

HR - CROATIA

Number of pages

14

Pages from-to

165-178

UT code for WoS article

000377053400009

EID of the result in the Scopus database

2-s2.0-84971543394