Complement activation dramatically accelerates blood plasma fouling on antifouling poly(2-hydroxyethyl methacrylate) brush surfaces

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00555739" target="_blank" >RIV/61389013:_____/22:00555739 - isvavai.cz</a>

Alternative codes found

RIV/00023736:_____/22:00013361

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1002/mabi.202100460" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/mabi.202100460</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mabi.202100460" target="_blank" >10.1002/mabi.202100460</a>

Result language

angličtina

Original language name

Complement activation dramatically accelerates blood plasma fouling on antifouling poly(2-hydroxyethyl methacrylate) brush surfaces

Original language description

Non-specific protein adsorption (fouling) triggers a number of deleterious events in the application of biomaterials. Antifouling polymer brushes successfully suppress fouling, however for some coatings an extremely high variability of fouling for different donors remains unexplained. The authors report that in the case of poly(2-hydroxyethyl methacrylate) (poly(HEMA)) this variability is due to the complement system activation that causes massive acceleration in the fouling kinetics of blood plasma. Using plasma from various donors, the fouling kinetics on poly(HEMA) is analyzed and correlated with proteins identified in the deposits on the surface and with the biochemical compositions of the plasma. The presence of complement components in fouling deposits and concentrations of C3a in different plasmas indicate that the alternative complement pathway plays a significant role in the fouling on poly(HEMA) through the “tick-over” mechanism of spontaneous C3 activation. The generated C3b binds to the poly(HEMA) surface and amplifies complement activation locally. Heat-inactivated plasma prevents accelerated fouling kinetics, confirming the central role of complement activation. The results highlight the need to take into account the variability between individuals when assessing interactions between biomaterials and blood plasma, as well as the importance of the mechanistic insight that can be gained from protein identification.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/GA20-10845S" target="_blank" >GA20-10845S: Blood plasma individual variability and pathophysiology and their influence on the interactions with synthetic antifouling surfaces</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Macromolecular Bioscience

ISSN

1616-5187

e-ISSN

1616-5195

Volume of the periodical

22

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

2100460

UT code for WoS article

000738641200001

EID of the result in the Scopus database

2-s2.0-85122330307