Antibiotic-loaded amphiphilic chitosan nanoparticles target macrophages and kill an intracellular pathogen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00559145" target="_blank" >RIV/61389013:_____/22:00559145 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/22:00559145 RIV/44555601:13440/22:43896982 RIV/60162694:G33__/22:N0000002 RIV/62157124:16170/22:43879895
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/smll.202201853" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/smll.202201853</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/smll.202201853" target="_blank" >10.1002/smll.202201853</a>
Alternative languages
Result language
angličtina
Original language name
Antibiotic-loaded amphiphilic chitosan nanoparticles target macrophages and kill an intracellular pathogen
Original language description
In this work, levofloxacin (LVX), a third-generation fluoroquinolone antibiotic, is encapsulated within amphiphilic polymeric nanoparticles of a chitosan-g-poly(methyl methacrylate) produced by self-assembly and physically stabilized by ionotropic crosslinking with sodium tripolyphosphate. Non-crosslinked nanoparticles display a size of 29 nm and a zeta-potential of +36 mV, while the crosslinked counterparts display 45 nm and +24 mV, respectively. The cell compatibility, uptake, and intracellular trafficking are characterized in the murine alveolar macrophage cell line MH-S and the human bronchial epithelial cell line BEAS-2B in vitro. Internalization events are detected after 10 min and the uptake is inhibited by several endocytosis inhibitors, indicating the involvement of complex endocytic pathways. In addition, the nanoparticles are detected in the lysosomal compartment. Then, the antibacterial efficacy of LVX-loaded nanoformulations (50% w/w drug content) is assessed in MH-S and BEAS-2B cells infected with Staphylococcus aureus and the bacterial burden is decreased by 49% and 46%, respectively. In contrast, free LVX leads to a decrease of 8% and 5%, respectively, in the same infected cell lines. Finally, intravenous injection to a zebrafish larval model shows that the nanoparticles accumulate in macrophages and endothelium and demonstrate the promise of these amphiphilic nanoparticles to target intracellular infections.n
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Small
ISSN
1613-6810
e-ISSN
1613-6829
Volume of the periodical
18
Issue of the periodical within the volume
28
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
2201853
UT code for WoS article
000809630500001
EID of the result in the Scopus database
2-s2.0-85131720748