Modeling receptor-mediated endocytosis of polymer-functionalized iron oxide nanoparticles by human macrophages

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F11%3A00375490" target="_blank" >RIV/68378271:_____/11:00375490 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.biomaterials.2010.08.111" target="_blank" >http://dx.doi.org/10.1016/j.biomaterials.2010.08.111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biomaterials.2010.08.111" target="_blank" >10.1016/j.biomaterials.2010.08.111</a>

Result language

angličtina

Original language name

Modeling receptor-mediated endocytosis of polymer-functionalized iron oxide nanoparticles by human macrophages

Original language description

Although systemically applied nanoparticles are quickly taken up by phagocytic cells, mainly macrophages, the interactions between engineered nanoparticles and macrophages are still not well defined. We therefore analyzed the uptake of diagnostically used carboxydextran-coated superparamagnetic iron oxide nanoparticles of 60 nm (SPIO) and 20 nm (USPIO) by human macrophages. By pharmacological and in vitro knockdown approaches, the principal uptake mechanism for both particles was identified as clathrin-mediated, scavenger receptor A-dependent endocytosis. We developed a mathematical model of the uptake process that allows determination of key parameters of endocytosis, including the rate of uptake, the number of nanoparticles per cell in saturation, the mean uptake time, and the correlation between the number of internalized nanoparticles and their extracellular concentration. The calculated parameters correlate well with experimental data obtained by confocal microscopy.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BM - Solid-state physics and magnetism

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomaterials

ISSN

0142-9612

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

547-555

UT code for WoS article

000285401500023

EID of the result in the Scopus database

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