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pH-induced morphological reversible transition from microparticles to vesicles for effective bacteria entrapment

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F24%3A00600337" target="_blank" >RIV/61389013:_____/24:00600337 - isvavai.cz</a>

  • Alternative codes found

    RIV/60162694:G44__/25:00564001 RIV/00179906:_____/24:10488264

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0014305724007729?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014305724007729?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.eurpolymj.2024.113511" target="_blank" >10.1016/j.eurpolymj.2024.113511</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    pH-induced morphological reversible transition from microparticles to vesicles for effective bacteria entrapment

  • Original language description

    Polymer particles with stimuli-responsive properties offer promising applications in healthcare, chemical reactors, development of artificial cells and organelles, as well as in the entrapment of bacteria. In this study, a novel biocompatible, biodegradable, and pH-responsive diblock copolymer based on polylactide (PLA) and poly(2-(diisopropylamino)ethyl methacrylate) (PDPA) was synthesized via a metal-free one-pot/simultaneous ring-opening polymerization (ROP) and reversible addition-fragmentation chain transfer (RAFT) approach (ROP/RAFT). This copolymer was then employed to produce highly monodisperse microparticles using microfluidics droplet generation technique. Utilizing confocal microscopy imaging, a reversible pH-induced morphological transition from microparticles to vesicle-like structures was observed at pH 5.1. The reversible shift in morphology from microparticles to giant vesicles (Vs) in acidic environments is triggered by the protonation of amino groups of the PDPA block, rendering vesicle surfaces positively charged − an advantageous feature for attracting and engulfing negatively charged bacteria. Initial validation involved electrostatic interactions with negatively charged latex resin beads followed by assessing interaction capabilities with gram-negative bacteria, Escherichia coli (E. coli). Additionally, the reversible morphological transition of microparticles-to-vesicles was employed to study drug release at different pHs. This approach proven to be a promising strategy for targeted drug delivery and bacteria entrapment using smart pH-responsive microparticles.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10404 - Polymer science

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2024

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    European Polymer Journal

  • ISSN

    0014-3057

  • e-ISSN

    1873-1945

  • Volume of the periodical

    221

  • Issue of the periodical within the volume

    11 December

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    12

  • Pages from-to

    113511

  • UT code for WoS article

    001349898800001

  • EID of the result in the Scopus database

    2-s2.0-85207953784