Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L).

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F02%3A56023014" target="_blank" >RIV/61389030:_____/02:56023014 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L).

Original language description

Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theoretical and Applied Genetics

ISSN

0040-5752

e-ISSN

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Volume of the periodical

104

Issue of the periodical within the volume

N/A

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

1362-1372

UT code for WoS article

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EID of the result in the Scopus database

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