Induction of RNA-mediated resistance to papaya ringspot virus type W
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00305977" target="_blank" >RIV/61389030:_____/07:00305977 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Induction of RNA-mediated resistance to papaya ringspot virus type W
Original language description
Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the35S CaMV promoter (pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenictines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these tines with PRSV-W revealed that whereas the fine containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic
Czech name
Indukce rezistence řízené RNA k viru kroužkovitosti papáje typu W
Czech description
Agrotransformace Cucumis melo binárním vektorem nesoucím inverzní repetitivní sekvenci genu pro obalový protein viru kroužovitosti papáje typu W byla použita pro cílené navození rezistence C. melo k tomuto viru. Byly získány čtyři rezistentní linie, které akumulovaly siRNA a tedy rezistence byla řízena RNA na bázi PTGS.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biochemistry and Molecular Biology
ISSN
1225-8687
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
3
Country of publishing house
SG - SINGAPORE
Number of pages
8
Pages from-to
404-411
UT code for WoS article
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EID of the result in the Scopus database
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