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Induction of RNA-mediated resistance to papaya ringspot virus type W

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00305977" target="_blank" >RIV/61389030:_____/07:00305977 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Induction of RNA-mediated resistance to papaya ringspot virus type W

  • Original language description

    Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the35S CaMV promoter (pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenictines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these tines with PRSV-W revealed that whereas the fine containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic

  • Czech name

    Indukce rezistence řízené RNA k viru kroužkovitosti papáje typu W

  • Czech description

    Agrotransformace Cucumis melo binárním vektorem nesoucím inverzní repetitivní sekvenci genu pro obalový protein viru kroužovitosti papáje typu W byla použita pro cílené navození rezistence C. melo k tomuto viru. Byly získány čtyři rezistentní linie, které akumulovaly siRNA a tedy rezistence byla řízena RNA na bázi PTGS.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biochemistry and Molecular Biology

  • ISSN

    1225-8687

  • e-ISSN

  • Volume of the periodical

    40

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    SG - SINGAPORE

  • Number of pages

    8

  • Pages from-to

    404-411

  • UT code for WoS article

  • EID of the result in the Scopus database