Resistance of transgenic papaya plants to Papaya Ringspot Virus, Thai isolate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00306878" target="_blank" >RIV/61389030:_____/07:00306878 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Resistance of transgenic papaya plants to Papaya Ringspot Virus, Thai isolate

Original language description

The coat protein gene isolated from Papaya ringspot virus, Thai isolate, was used to generate transgenic papayas. A binary vector containing the coat protein gene under the control of a 35S promoter, was constructed and transformed into somatic embryos of papaya cultivar Khak Dum by microprojectile bombardment. Eight transgenic lines were identified from 1980 bombarded calli of papaya somatic embryos under kanamycin selection. Integration of the transferred genes into kanamycin resistant papaya calli was verified by PCR amplification of the coat protein gene, GUS assays and Southern blot hybridization. Although the coat protein gene was detected in all transgenic lines, only line G2 was found to be highly resistant to virus. This resistant line showedhigh degree of rearrangement of the inserted coat protein expression cassette while the coat protein gene itself had a deletion of 166 bp on the 3' end of its sequence. Although the transcription of the coat protein gene was detected in a

Czech name

Resistence transgení papáje k thajskému izolátu viru kroužkovitosti papáje

Czech description

Byla generována papája rezistentní k viru kroužkovitosti papáje (PRSV) a to biolistickou transformací somatických embryí genem pro obalový protein (CP) tohoto viru pod kontrolou promotoru 35S. Z celkem 1980 embryogeních kalusů bylo pouze 8 vyselektovánona půdě s kanamycinem. U všech těchto linii byl detegován CP pomocí PCR a Southern blot hybridizace. Avšak pouze linie G2 byla 100 % rezistentní k viru PRSV.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

333-339

UT code for WoS article

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EID of the result in the Scopus database

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