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Post-transcriptional gene silencing is involved in resistance of transgenic papayas to Papaya Ringspot Virus

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00307205" target="_blank" >RIV/61389030:_____/07:00307205 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Post-transcriptional gene silencing is involved in resistance of transgenic papayas to Papaya Ringspot Virus

  • Original language description

    Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing(PTGS).

  • Czech name

    Post-transkripční útlum genů se účastní mechanismu rezistence transgenních papají k viru kroužkovitosti papáje

  • Czech description

    Byly analyzovány transgenní papáje s vneseným genem obalového proteinu viru (CP) kroužkovitosti papáje. Linie G2, která obsahuje aberantní DNA CP a která akumulovala siRNA, byla v T0 generaci rezistentní k tomuto viru. Analýzou by potvrzeno, že tato rezistence je řízené RNA na základě post-transkripčního útlumu genů.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biologia Plantarum

  • ISSN

    0006-3134

  • e-ISSN

  • Volume of the periodical

    51

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    4

  • Pages from-to

    517-520

  • UT code for WoS article

  • EID of the result in the Scopus database