Post-transcriptional gene silencing is involved in resistance of transgenic papayas to Papaya Ringspot Virus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00307205" target="_blank" >RIV/61389030:_____/07:00307205 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Post-transcriptional gene silencing is involved in resistance of transgenic papayas to Papaya Ringspot Virus
Original language description
Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing(PTGS).
Czech name
Post-transkripční útlum genů se účastní mechanismu rezistence transgenních papají k viru kroužkovitosti papáje
Czech description
Byly analyzovány transgenní papáje s vneseným genem obalového proteinu viru (CP) kroužkovitosti papáje. Linie G2, která obsahuje aberantní DNA CP a která akumulovala siRNA, byla v T0 generaci rezistentní k tomuto viru. Analýzou by potvrzeno, že tato rezistence je řízené RNA na základě post-transkripčního útlumu genů.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biologia Plantarum
ISSN
0006-3134
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
517-520
UT code for WoS article
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EID of the result in the Scopus database
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