Isolation of recombinant cysteine dioxygenase protein from Trichophyton mentagrophytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00368595" target="_blank" >RIV/61389030:_____/11:00368595 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/11:10213118 RIV/61989592:15110/11:10213118
Result on the web
<a href="http://dx.doi.org/10.1111/j.1439-0507.2010.01948.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0507.2010.01948.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1439-0507.2010.01948.x" target="_blank" >10.1111/j.1439-0507.2010.01948.x</a>
Alternative languages
Result language
angličtina
Original language name
Isolation of recombinant cysteine dioxygenase protein from Trichophyton mentagrophytes
Original language description
Cysteine dioxygenase (CDO, EC 1.13.11.20) catalyses the oxygenation of cysteine to cysteine sulphinic acid leading to the production of sulphite, sulphate and taurine as the final metabolites of cysteine catabolism. Keratinolytic fungi secrete sulphite and sulphate to reduce disulphide bridges in host tissue keratin proteins as the first step of keratinolysis. In the present study, we describe the identification of cDNA, as well as expression and characterisation of recombinant CDO protein from Trichophyton mentagrophytes. The cDNA was amplified using primers designed on the basis of high conservancy CDO regions identified in other fungi. PCR product was cloned and sequenced. Recombinant CDO was expressed in Escherichia colt, and affinity purified andidentified by matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry (MALDI-TOF MS). Enzyme activity was assayed by monitoring the production of cysteine sulphinate using mass spectrometry.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA301%2F08%2F1649" target="_blank" >GA301/08/1649: Modulation of cell division of normal and cancer cells by cyclin-dependent kinase inhibitors</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mycoses
ISSN
0933-7407
e-ISSN
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Volume of the periodical
54
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
"E456"-"E462"
UT code for WoS article
000294878900118
EID of the result in the Scopus database
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