Exposition of dermatophyte Trichophyton mentagrophytes to L-cystine induces expression and activation of cysteine dioxygenase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33149992" target="_blank" >RIV/61989592:15310/14:33149992 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/14:33149992

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/myc.12220/abstract;jsessionid=AFA87338B457A732EB91BD02F75AA0D4.f04t01" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/myc.12220/abstract;jsessionid=AFA87338B457A732EB91BD02F75AA0D4.f04t01</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/myc.12220" target="_blank" >10.1111/myc.12220</a>

Result language

angličtina

Original language name

Exposition of dermatophyte Trichophyton mentagrophytes to L-cystine induces expression and activation of cysteine dioxygenase

Original language description

Cysteine dioxygenase (CDO) is involved in regulation of intracellular cysteine levels by catabolising the cysteine to sulphite and sulphate. In keratinolytic fungi, sulphite is actively excreted to reduce disulphide bridges in keratin before its enzymatic degradation. The pathogenicity role of CDO was confirmed in cysteine-hypersensitive and growth-defective DCdo mutant of Arthroderma benhamiae on hair and nails. We analysed the CDO expression regulation in T. mentagrophytes (anamorph of A. benhamiae) mycelia by determining the Cdo mRNA and CDO protein levels and by analysing the proportion of two molecular forms of CDO in response to L-cystine exposure. Cdo mRNA levels in mycelia lysates were detected by reverse-transcription real-time polymerase chain reaction and CDO protein by western blot using mouse CDO-specific hyperimmune serum. The Cdo mRNA level increased gradually 2.5-4.5 h after exposure of the mycelium to L-cystine. The CDO protein, detected as two bands of different mobil

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mycoses

ISSN

0933-7407

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

"672?678"

UT code for WoS article

000345648900005

EID of the result in the Scopus database

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