New insights into auxin metabolism in Bradyrhizobium japonicum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00493452" target="_blank" >RIV/61389030:_____/18:00493452 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/18:73592386
Result on the web
<a href="http://dx.doi.org/10.1016/j.resmic.2018.04.002" target="_blank" >http://dx.doi.org/10.1016/j.resmic.2018.04.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.resmic.2018.04.002" target="_blank" >10.1016/j.resmic.2018.04.002</a>
Alternative languages
Result language
angličtina
Original language name
New insights into auxin metabolism in Bradyrhizobium japonicum
Original language description
Bacterial metabolism of phytohormones includes several processes such as biosynthesis, catabolism, conjugation, hydrolysis and homeostatic regulation. However, only biosynthesis and occasionally catabolism are studied in depth in microorganisms. In this work, we evaluated and reconsidered IAA metabolism in Bradyrhizobium japonicum E109, one of the most widely used strains for soybean inoculation around the world. The genomic analysis of the strain showed the presence of several genes responsible for IAA biosynthesis, mainly via indole-3-acetonitrile (IAN), indole-3-acetamide (LAM) and tryptamine (TAM) pathways. However, in vitro experiments showed that IAA is not accumulated in the culture medium in significant amounts. On the contrary, a strong degradation activity was observed after exogenous addition of 0.1 mM of IAA, IBA or NAA to the medium. B. japonicum E109 was not able to grow in culture medium containing IAA as a sole carbon source. In YEM medium, the bacteria degraded IAA and hydrolyzed amino acid auxin conjugates with alanine (IAAIa), phenylalanine (LAPhe), and leucine (LAPhe), releasing IAA which was quickly degraded. Finally, the presence of exogenous IAA induced physiological changes in the bacteria such as increased biomass and exopolysaccharide production, as well as infection effectiveness and symbiotic behavior in soybean plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Research in Microbiology
ISSN
0923-2508
e-ISSN
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Volume of the periodical
169
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
313-323
UT code for WoS article
000439104700006
EID of the result in the Scopus database
2-s2.0-85047182231