Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00503477" target="_blank" >RIV/61389030:_____/18:00503477 - isvavai.cz</a>

Alternative codes found

RIV/67985939:_____/18:00492148

Result on the web

<a href="http://dx.doi.org/10.1007/s00606-017-1476-x" target="_blank" >http://dx.doi.org/10.1007/s00606-017-1476-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00606-017-1476-x" target="_blank" >10.1007/s00606-017-1476-x</a>

Result language

angličtina

Original language name

Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae

Original language description

Phylogenetic and barcoding studies usually use fresh plant tissues as sources of DNA and have successfully amplified DNA for various loci. The use of dried samples, however, is often necessary due to the frequent inaccessibility of fresh rare plants or their parts for genetic analyses or barcoding. The difficulty in obtaining amplifiable DNA is a major restriction of the use of herbarium specimens for DNA analyses. Recent study has highlighted the crucial issues for comparing herbarium and fresh plants for barcoding. We analysed the performance of samples of the family Juncaceae from various herbarium specimens of different ages with fresh plant material in PCRs and the sequences of seven loci (rbcL, rpoC1, trnL-F intergenic spacer, trnL intron, and psbA-trnH from chloroplast DNA, atp1 from mitochondrial DNA, and ITS1-5.8S-ITS2 from nuclear DNA) using a combination of 28 primers. The herbarium specimens amplified well and may thus be successfully applied for both phylogenetic analyses and barcoding for the Juncaceae family. Amplifying DNA was more difficult from dried herbarium specimens than fresh samples but could be successful in most cases when appropriate internal primers were designed or methods were optimised. Using the set of universal primers recommended by the Consortium for the Barcode of Life and designing specific primers for a particular group of interest were both useful. Specimen age and amplicon length had limited detrimental effects on amplification success for most of the Juncaceae loci tested.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Systematics and Evolution

ISSN

0378-2697

e-ISSN

—

Volume of the periodical

304

Issue of the periodical within the volume

2

Country of publishing house

AT - AUSTRIA

Number of pages

14

Pages from-to

281-294

UT code for WoS article

000422970000011

EID of the result in the Scopus database

2-s2.0-85035348604