DNA extraction from herbarium specimens

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F14%3A00436267" target="_blank" >RIV/67985939:_____/14:00436267 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-62703-767-9_4" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-767-9_4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-767-9_4" target="_blank" >10.1007/978-1-62703-767-9_4</a>

Result language
angličtina
Original language name
DNA extraction from herbarium specimens
Original language description
With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen and chemical treatmentof the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols have been described.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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