The effect of different DNA isolation protocols and AFLP fingerprinting optimizations on error rate estimates in the bryophyte Campylopus introflexus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10131722" target="_blank" >RIV/00216208:11310/12:10131722 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/12:00059386

Result on the web

<a href="http://www.oikos.ekol.lu.se/lindbergiapdfs/Lindbergia35.7-17.pdf" target="_blank" >http://www.oikos.ekol.lu.se/lindbergiapdfs/Lindbergia35.7-17.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The effect of different DNA isolation protocols and AFLP fingerprinting optimizations on error rate estimates in the bryophyte Campylopus introflexus

Original language description

Amplified fragment length polymorphism (AFLP) has become a standard method for investigating genetic variation in plants. Nevertheless, only a few applications in bryophytes have been published and there is still a need to optimize the method. We optimized DNA isolation and AFLP protocols for Campylopus introflexus (Hedw.) Brid. As DNA quality is crucial for successful AFLP analysis, three different DNA extraction protocols were compared and the Invisorb Plant Mini Kit produced the highest DNA amount (15, 38 and 47 ng ?l-1 for dry, fresh and new cultivated tissues, respectively) and purity. Newly grown stems gave the purest DNA (absorbance at the wavelengths of 260 nm and 280 nm was 1.86). However, banding patterns obtained from dry herbarium specimens(up to two years old) corresponded with those from fresh material at a similarity level of 94.71%. The replicability of AFLP profiles was not dependent on the way plants were stored. We compared commercial kits and the influence of modif

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Lindbergia

ISSN

0105-0761

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

22.2.

Country of publishing house

SE - SWEDEN

Number of pages

11

Pages from-to

7-17

UT code for WoS article

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EID of the result in the Scopus database

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