Concurrent overexpression of amino acid permease AAP1 (3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00549508" target="_blank" >RIV/61389030:_____/21:00549508 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/21:73610162

Result on the web

<a href="http://doi.org/10.1071/FP21011" target="_blank" >http://doi.org/10.1071/FP21011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1071/FP21011" target="_blank" >10.1071/FP21011</a>

Result language

angličtina

Original language name

Concurrent overexpression of amino acid permease AAP1 (3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels

Original language description

Using pea as our model crop, we sought to understand the regulatory control over the import of sugars and amino acids into the developing seeds and its importance for seed yield and quality. Transgenic peas simultaneously overexpressing a sucrose transporter and an amino acid transporter were developed. Pod walls, seed coats, and cotyledons were analysed separately, as well as leaves subtending developing pods. Sucrose, starch, protein, free amino acids, and endogenous cytokinins were measured during development. Temporal gene expression analyses (RT-qPCR) of amino acid (AAP), sucrose (SUT), and SWEET transporter family members, and those from cell wall invertase, cytokinin biosynthetic (IPT) and degradation (CKX) gene families indicated a strong effect of the transgenes on gene expression. In seed coats of the double transgenics, increased content and prolonged presence of cytokinin was particularly noticeable. The transgenes effectively promoted transition of young sink leaves into source leaves. We suggest the increased flux of sucrose and amino acids from source to sink, along with increased interaction between cytokinin and cell wall invertase in developing seed coats led to enhanced sink activity, resulting in higher cotyledon sucrose at process pea harvest, and increased seed number and protein content at maturity.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Functional Plant Biology

ISSN

1445-4408

e-ISSN

1445-4416

Volume of the periodical

48

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

889-904

UT code for WoS article

000653744600001

EID of the result in the Scopus database

2-s2.0-85106900009