Cytokinin oxidase/dehydrogenase inhibitors: outlook for selectivity and high efficiency

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00562506" target="_blank" >RIV/61389030:_____/22:00562506 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15640/22:73616672 RIV/61989592:15310/22:73616672

Result on the web

<a href="https://doi.org/10.1093/jxb/erac201" target="_blank" >https://doi.org/10.1093/jxb/erac201</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erac201" target="_blank" >10.1093/jxb/erac201</a>

Result language

angličtina

Original language name

Cytokinin oxidase/dehydrogenase inhibitors: outlook for selectivity and high efficiency

Original language description

Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation of cytokinins in plants, and this effect can be exploited in agriculture and in plant tissue culture. In this study, we examine the structure–activity relationship of two series of CKX inhibitors based on diphenylurea. The compounds of Series I were derived from the recently published CKX inhibitors 3TFM-2HM and 3TFM-2HE, and we identified key substituents with increased selectivity for maize ZmCKX1 and ZmCKX4a over AtCKX2 from Arabidopsis. Series II contained compounds that further exceled in CKX inhibitory activity as well as in the ease of their synthesis. The best inhibitors exhibited half-maximal inhibitory concentration (IC50) values in low nanomolar ranges with ZmCKX1 and especially with ZmCKX4a, which is generally more resistant to inhibition. The activity of the key compounds was verified in tobacco and lobelia leaf-disk assays, where N6-isopentenyladenine was protected from degradation and promoted shoot regeneration. All the prepared compounds were further tested for toxicity against Caenorhabditis elegans, and the assays revealed clear differences in toxicity between compounds with and without a hydroxyalkyl group. In a broader perspective, this work increases our understanding of CKX inhibition and provides a more extensive portfolio of compounds suitable for agricultural and biotechnological research.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

1460-2431

Volume of the periodical

73

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

4806-4817

UT code for WoS article

000808214600001

EID of the result in the Scopus database

2-s2.0-85137382315