Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00600679" target="_blank" >RIV/61389030:_____/24:00600679 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15640/24:73625945 RIV/61989592:15310/24:73625945 RIV/00216208:11310/24:10497194
Result on the web
<a href="https://doi.org/10.1093/jxb/erae239" target="_blank" >https://doi.org/10.1093/jxb/erae239</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erae239" target="_blank" >10.1093/jxb/erae239</a>
Alternative languages
Result language
angličtina
Original language name
Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice
Original language description
Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure-activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (identified as 82) activated a cytokinin primary response gene, ARR5:GUS, and a cytokinin sensor, TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the up-regulation of cytokinin signalling. Overall, this study identifies highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
1460-2431
Volume of the periodical
75
Issue of the periodical within the volume
16
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
4873-4890
UT code for WoS article
001268361800001
EID of the result in the Scopus database
2-s2.0-85202546213