Exploring the crop epigenome: a comparison of DNA methylation profiling techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575584" target="_blank" >RIV/61389030:_____/23:00575584 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43510/23:43923681

Result on the web

<a href="https://doi.org/10.3389/fpls.2023.1181039" target="_blank" >https://doi.org/10.3389/fpls.2023.1181039</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2023.1181039" target="_blank" >10.3389/fpls.2023.1181039</a>

Result language

angličtina

Original language name

Exploring the crop epigenome: a comparison of DNA methylation profiling techniques

Original language description

Epigenetic modifications play a vital role in the preservation of genome integrity and in the regulation of gene expression. DNA methylation, one of the key mechanisms of epigenetic control, impacts growth, development, stress response and adaptability of all organisms, including plants. The detection of DNA methylation marks is crucial for understanding the mechanisms underlying these processes and for developing strategies to improve productivity and stress resistance of crop plants. There are different methods for detecting plant DNA methylation, such as bisulfite sequencing, methylation-sensitive amplified polymorphism, genome-wide DNA methylation analysis, methylated DNA immunoprecipitation sequencing, reduced representation bisulfite sequencing, MS and immuno-based techniques. These profiling approaches vary in many aspects, including DNA input, resolution, genomic region coverage, and bioinformatics analysis. Selecting an appropriate methylation screening approach requires an understanding of all these techniques. This review provides an overview of DNA methylation profiling methods in crop plants, along with comparisons of the efficacy of these techniques between model and crop plants. The strengths and limitations of each methodological approach are outlined, and the importance of considering both technical and biological factors are highlighted. Additionally, methods for modulating DNA methylation in model and crop species are presented. Overall, this review will assist scientists in making informed decisions when selecting an appropriate DNA methylation profiling method.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Volume of the periodical

14

Issue of the periodical within the volume

MAY 30

Country of publishing house

CH - SWITZERLAND

Number of pages

24

Pages from-to

1181039

UT code for WoS article

001007263700001

EID of the result in the Scopus database

2-s2.0-85161895018