Anti-inflammatory and antiproliferative activity of Helichrysum odoratissimum Sweet. against lung cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00601085" target="_blank" >RIV/61389030:_____/24:00601085 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.sajb.2024.01.056" target="_blank" >https://doi.org/10.1016/j.sajb.2024.01.056</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2024.01.056" target="_blank" >10.1016/j.sajb.2024.01.056</a>

Result language

angličtina

Original language name

Anti-inflammatory and antiproliferative activity of Helichrysum odoratissimum Sweet. against lung cancer

Original language description

Lung cancer remains the top killing cancer worldwide despite advances in treatment. Seven ethanolic plant extracts were selected and evaluated for their antiproliferative activity against the two main types of lung cancers: non-small cell (A549) and small cell lung cancer cells (SHP-77). An ethanolic extract of Helichrysum odoratissimum Sweet (HO) showed significant antiproliferative activity against lung cancer, with a fifty percent inhibitory concentration (IC50) of 83.43 +/- 1.60 mu g/mL (A549), 49.46 +/- 0.48 mu g/mL (SHP-77) and 50.71 +/- 2.27 mu g/mL, against normal lung epithelial cells (MRC-5), resulting in a selectivity index (SI) value of 0.61 on A549 cells and 1.03 on SHP-77 cells, which was compared to the positive drug control, actinomycin D where the SI values were found to be 2 and 0.25 against A549 and SHP-77 cells, respectively. Against murine macrophages (RAW 264.7) and hepatocytes (HepG2), the HO ethanolic extract showed IC50 values of 60.15 +/- 1.98 mu g/mL and 23.61 +/- 1.06 mu g/mL, respectively. Microscopy showed that the HO ethanolic extract induced apoptosis in the A549 and HepG2 cells at 50 mu g/mL and 300 mu g/mL, respectively. The HO ethanolic extract, furthermore, inhibited the pro-inflammatory enzymes, cyclooxygenase 2 (COX-2) and 5-lipoxygenase (5-LOX) with IC50 values of 7.94 +/- 3.84 mu g/mL and 2.08 +/- 1.35 mu g/mL, respectively, whereas the positive controls Ibuprofen (COX-2) and Zileuton (5-LOX) showed IC50 values of 0.85 +/- 0.14 mu g/mL and 0.06 +/- 0.05 mu g/mL, respectively. The activity of NAD(P)H quinone oxidoreductase-1 (NQO1), which is a direct target of nuclear factor erythroid-2-related factor-2 (NRF2), was significantly inhibited in the A549 cells by the HO ethanolic extract (at 125 mu g/mL) when compared to the positive control, brusatol (at 500 nM). Using the ex ovo yolk sac membrane (YSM) assay, the HO ethanolic extract (at 18.5 mu g/egg) showed a 31.65 +/- 12.80% inhibition of blood vessel formation. This is the first report of the noteworthy antiproliferative activity of the HO ethanolic extract on lung cancer cells including its potential to target several enzymes associated with inflammation and therefore, should be considered for further analysis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

South African Journal of Botany

ISSN

0254-6299

e-ISSN

1727-9321

Volume of the periodical

166

Issue of the periodical within the volume

MAR

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

525-538

UT code for WoS article

001177291000001

EID of the result in the Scopus database

2-s2.0-85184072825